Solutions for Functional Proteomics

How KX Array™ Technology Works

Surface Plasmon Resonance imaging (SPRi) is an advanced optical technology that measures refractive index changes near or on a sensor surface. When light is reflected off a thin gold layer coated on glass at a certain angle of incidence, energy is transferred to plasmons on the surface of the gold layer causing them to resonate and reduce the amount of reflected light.

SPR Diagram

This surface plasmon resonance and the amount of reflected light is very sensitive to refractive index changes occurring on the surface of the gold due to changes in mass. It is these changes in reflected light intensities over time that are used to quantify the interaction of biomolecules.

SPR Sensorgram showing changes in light intensity against timeAs sample molecules (analytes) pass over the gold coated surface and bind to molecules immobilized to that surface (ligand), the changes in reflected light intensity are recorded and displayed in what is known as a “sensorgram”. Increased binding or association results in a rising curve while dissociation results in a declining curve. When measured over time, the association and dissociation rates as well as the maximum change in intensity can be used to calculate affinity and concentrations.

Using proprietary data analysis software developed by Plexera® LLC, the relative affinities of thousands of target biomolecules for multiple analytes can quickly be calculated providing a faster, more cost effective, and accelerated method for the discovery of new biomolecules such as antibodies and biomarkers.

Protein array and sensorgrams

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