Lectin Array Chip Kit
The Plexera Lectin Array Chip Kit — providing a convenient way for users to perform high throughput biomarker screening, kinetic determination, structural identification, and label-free characterization of glycoproteins in real time.Introduction
Glycosylation is one of the most common post-translational modifications that occur naturally in proteins. The dysregulation of glycosylation is associated with a wide range of diseases. Thus, the study of the glycome holds the key to new pathological biomarker discoveries. The combination of Plexera's PlexArray® SPRi instrument and high density Nanocapture® Lectin Array Chip provides our customers a unique high-throughput platform for glycoprotein screening. This technology takes advantage of the key roles lectins play in many biological pathways, and helps scientists elucidate the important dynamic functions of the glycome.
The Lectin Array platform includes 41 different lectins from seven glycan binding moieties. Three technical replicates of lectins and controls are printed on the same chip, and 270 reactions can be monitored simultaneously. The Lectin Array Nanocapture chip has been tested for long term stability and resistance to a multitude of regeneration solutions and cycles. It can be used many times for binding assays without the need for replacement. Additional details and supporting documentation and information are available in the Lectin Array Application Note and Poster.
Kit Components
The Lectin Array Kit packages contain either one or two array chips, printed with lectins that recognize seven different glycan structures: acetygalactosamiyl, mannosyl, galactosyl, fucosyl, sialic acid, acetyglucosamine, and neu5Ac. The kit also contains either two or four reference analytes for the purpose of demonstrating a multitude of lectin binding abilities and practical applications. The appropriate buffers are supplied as well.
Lectin Array Applications
Glycan structure determination from purified transferrin analysis
Glycoform Characterization
- Kinetics determination
- Glycan structure determination from a single purified glycoprotein
- Low concentration glycoprotein signal amplification
- Concentration determination
Glycoform profile from human serum