Applications / AI Protein Design

Measured kinetic data
for AI-designed proteins.

Move from designed sequences to comparable experimental data through one expression, purification and SPRi workflow.

10–14 days
Sequence to report
KD · kon · koff
Affinity and kinetics
3 × 8
Replicates × concentrations

THE DATA GAP

Design models need
measured biological evidence.

Sequence and structural predictions do not capture the full binding response. Consistent kinetic measurements show whether a design binds, how quickly it associates and how long the interaction persists.

  • 01Train

    Add measured affinity and rate constants to model-development datasets.

  • 02Rank

    Compare designed variants within the same experimental framework.

  • 03Validate

    Confirm binding behavior before advancing selected constructs.

  • 04Iterate

    Return experimental results to the next design cycle.

DESIGN–MEASURE LOOP

From sequence library
to kinetic dataset.

  1. 01Submit sequences

    Provide designed protein sequences, target information and validation goals.

  2. 02Express and purify

    Plexera produces ligand proteins and completes purification and QC.

  3. 03Measure by SPRi

    Run the defined interaction matrix across replicates and concentration series.

  4. 04Return the data

    Receive sensorgrams, fitted kinetics and a structured report.

DATA FOR MODELING AND DECISIONS

A dataset built
for comparison.

Use one consistent assay framework to connect sequence changes with measured binding behavior.

  • Concentration-resolved sensorgrams
  • KD, kon and koff
  • Replicate and fitting information
  • Ranked design performance
  • Structured experimental context