Project inputs
- Ligand protein sequences
- Analyte or target information
- Desired interaction matrix
- Experimental objectives
Services / Protein binding kinetics
From protein sequences
to kinetic data.
Submit your sequences and target information. Plexera handles protein expression, purification, NanoCapture chip preparation, SPRi measurement and complete data reporting in 10–14 days.
Discuss your projectRepresentative SPRi kinetic dataConcentration-resolved sensorgrams
- 10–14 days
- Sequence to report
- 3 replicates
- Per ligand
- 8 concentrations
- Full-affinity series
- KD · kon · koff
- Kinetic parameters
A COMPLETE WET-LAB SERVICE
You provide the sequences.
Plexera runs the wet lab.
Complete execution
- Protein expression, purification and QC
- NanoCapture chip layout and preparation
- SPRi measurement and kinetic fitting
- Data review and reporting
Comparable data
- Concentration-resolved sensorgrams
- KD, kon and koff
- Replicate and fitting information
- A structured experimental report
HIGH-THROUGHPUT CFPS
Parallel protein production,
built into the service.
Plexera converts DNA templates into purified ligand proteins in parallel using cell-free protein synthesis. Expression, purification and quality control are integrated with NanoCapture chip preparation and SPRi measurement.
VHHscFvIgGEngineered proteins
Sequence to purified ligand proteinFrom $69 / protein
SEQUENCE-TO-KINETICS WORKFLOW
From sequence to report in five stages.
Plexera manages the experimental workflow from project planning through kinetic data delivery.
- 01
Plan the experiment
Confirm sequences, molecular formats, controls and ligand–analyte pairings.
- 02
Express and purify
Produce ligand proteins, complete purification and perform quality control.
- 03
Prepare the chip
Immobilize purified proteins on NanoCapture with replicates and assay controls.
- 04
Measure by SPRi
Run analytes across the array in an eight-point concentration series.
- 05
Receive the data
Review sensorgrams, fitted kinetic parameters and the complete project report.
DATA DELIVERY
A complete kinetic dataset,
ready to compare.
Each project returns the experimental context needed to compare designs across one consistent assay framework.
EXPERIMENTAL REPORTSPRi / FULL AFFINITY
KDBinding affinity
konAssociation rate
koffDissociation rate
- Complete concentration-resolved sensorgrams
- Three-replicate measurement results
- Eight-point concentration series
- Kinetic fitting and quality-control information
- Structured experimental report
REPRESENTATIVE DATA
Binding strength and kinetics,
measured together.
Sensorgrams are fitted to return affinity, association and dissociation parameters across the interaction matrix. The kinetic distribution shows how designs separate by binding behavior within one consistent assay framework.
KD Affinitykon Associationkoff Dissociation
PROJECT ESTIMATE
Estimate your project
Enter the number of immobilized ligand proteins and analyte proteins to calculate an indicative project cost.
YOUR PROJECT
Define the interaction matrix
Each ligand is expressed and measured against every analyte in your project.
Ligand expression100 × $69$6,900
Binding interactionsLigands × analytes200
SPRi measurements200 × $10$2,000
Indicative totalUSD$8,900
This estimate includes ligand protein expression and full-affinity SPRi measurements. Analyte protein preparation, purification or sourcing is quoted separately.
MOLECULAR FORMATS
Designed for protein
and antibody programs.
- 01Proteins
- 02VHH
- 03scFv
- 04IgG
- 05Antigens
- 06Engineered binders
PROJECT QUESTIONS
Before your project starts
What information do I need to submit?
Send the ligand sequences, analyte or target information, desired interaction matrix and the questions the experiment should answer.
Are the ligand proteins purified?
Yes. The standard service includes protein expression, purification and quality control before NanoCapture chip preparation.
What if my analyte protein is not available?
Share the analyte format, sequence or preferred source with the Plexera team. Preparation, purification or sourcing requirements and pricing are confirmed during project planning.
What is included in the final report?
The report includes concentration-resolved sensorgrams, fitted KD, kon and koff, replicate results, fitting information and the experimental context.
START A PROJECT
Tell us what
you need to measure.
Share your sequence set, target information and expected interaction matrix. We will review the experimental plan with you.
sale@plexera.com