Technology

Three-Dimensional Surface Chemistry

The 3D Surface Chemistry Technology maximizes the utilization of microscopic three-dimensional space by constructing a "polymer brush" layer on the gold substrate used for SPRi detection. Built on this brush-like polymer structure, the platform incorporates polyethylene glycol (PEG) side chains to minimize non-specific adsorption. These side chains also serve as linkers for immobilized molecules, granting them a high degree of three-dimensional freedom similar to that in a solution environment.

Advantages

Compared with traditional two-dimensional surface chemistry, the three-dimensional surface chemistry of biochips has many advantages, which are mainly reflected in the following aspects:

Increased surface area

Higher loading capacity: The three-dimensional structure significantly increases the effective surface area of the chip, which can fix more biomolecules and improve detection sensitivity.
Enhanced signal strength: A larger surface area helps capture more target molecules, thereby enhancing the detection signal.

Improved sensitivity

Low concentration detection: The high loading capacity of the three-dimensional structure enables it to detect lower concentrations of biomolecules, which is suitable for trace analysis.
Signal amplification: The three-dimensional structure can be combined with a variety of signal amplification technologies to further improve detection sensitivity.

Enhanced specificity

Reduced nonspecific binding: The design of the three-dimensional structure can reduce nonspecific binding and improve the specificity of detection.
Optimized molecular orientation: The three-dimensional structure can better control the orientation of biomolecules and enhance their specific binding with target molecules.
Improved reaction kinetics

Faster reaction rate: The three-dimensional structure provides more reaction sites and accelerates the rate of interaction between biomolecules.
More efficient mass transfer: The three-dimensional structure helps to improve the mixing and diffusion efficiency of the sample solution and improve reaction kinetics.

Versatility

Multi-type detection: The three-dimensional structure is suitable for the detection of a variety of biomolecules, such as DNA, RNA, protein, cells, etc.
Diversified applications: It is widely used in gene expression analysis, protein interaction research, disease diagnosis and other fields.

Improve stability and repeatability

Enhance stability: The three-dimensional structure can provide a more stable fixed environment and reduce the shedding and denaturation of biomolecules.
Improve repeatability: The uniformity and consistency of the three-dimensional structure help improve the repeatability and reliability of detection