PLEXERA®

Products / SPRi system

PlexArray HT
Real-time interaction analysis at array scale.

An ultra-high-throughput, label-free SPR imaging platform combining high-density microarrays, automated fluidics and real-time analysis to measure KD, kon, koff, Rmax and binding specificity.

PlexArray HT SPRi system
PLEXARRAY HTSPRi / LABEL-FREE / REAL-TIME
3,840
Spots per chip
2 cm²
Detection area
400,000
Interaction curves per day, up to
KD · kon · koff · Rmax
Reported parameters

THE COMPLETE PLATFORM

Instrument, software and chips
in one SPRi system.

PlexArray HT connects experiment control, wide-field imaging, kinetic analysis and NanoCapture consumables in one coordinated workflow.

  1. 01PlexArray HT instrument

    Wide-field SPR imaging, precision fluidics and automated sample handling.

  2. 02Instrument control software

    Method setup, temperature control and experiment monitoring.

  3. 03Data analysis software

    Real-time extraction, kinetic fitting, replicate comparison and reporting.

  4. 04NanoCapture chips

    High-density microarrays with application-specific surface chemistry.

SURFACE PLASMON RESONANCE IMAGING

Monitor every illuminated spot
across the array.

PlexArray HT records refractive-index changes across the microarray as immobilized molecules interact with samples in solution. Wide-field SPR imaging turns those changes into real-time association and dissociation responses.

SPRi optical path and data-processing principle showing the light source, prism, microarray chip, image capture and kinetic analysis
SPRi optical path, wide-field image capture and parallel kinetic analysis

AUTOMATED EXPERIMENTS

Designed for repeatable,
unattended operation.

Automated sample handling and configurable fluidics support longer, more complex interaction experiments without continuous operator attention.

  • 0196-well plate autosampling

    Queue samples for automated injection.

  • 024°C sample control

    Maintain sample temperature during the run.

  • 03Automatic gradients

    Prepare and run concentration series.

  • 04Sample recovery

    Recover flow-through or eluate samples.

  • 05Unattended experiments

    Support overnight operation.

  • 06Pause and alarm handling

    Respond to abnormal operating conditions.

Front view of the PlexArray HT instrument
PlexArray HT instrument
PlexArray HT data analysis software interface
DATA ANALYSIS SOFTWAREImage review, curve extraction and compound-level information

CONTROL AND ANALYSIS

From method setup
to fitted kinetics.

Control the experiment, review imaging responses and move directly into kinetic fitting and report generation.

Real-time imagingRecord interaction responses and difference maps during the experiment.

Automatic fittingAnalyze more than 10,000 curves within 10 minutes under typical test conditions.

Multiple modelsSupport 1:1, 1:2, nonspecific adsorption and two-site binding models.

Report exportCompare array and experimental replicates, then export the report.

BUILT-IN EXPERIMENT MODES

Configure the assay
for the question.

MOLECULAR COVERAGE

One open platform.
Multiple molecular formats.

PlexArray HT supports interaction analysis across a broad range of molecular systems.

  • Proteins
  • Antibodies
  • VHH
  • Peptides
  • Small molecules
  • DNA / RNA
  • Glycans
  • Aptamers

TECHNICAL SPECIFICATIONS

System specifications

Typical performance depends on system configuration, assay conditions and curve-fitting settings.

Detection principleSurface plasmon resonance imaging (SPRi)
Reported parameterskon, koff, KD, Rmax, binding specificity
Microarray capacity3,840 spots / chip
Detection area2 cm²
Minimum molecular weight>150 Da
Association rate, typical103–107 M−1 s−1
Dissociation rate, typical10−6–10−3 s−1
Affinity range, typical10−12–10−3 M
Detection limit0.5 ng/cm²
Flow rate0.2–50 μL/s
Microplate temperature4°C to room temperature
Detection temperature10–40°C
Processing timeApproximately 20 minutes per sample
Daily outputUp to 400,000 interaction curves

NEXT-GENERATION FORMAT / COMING SOON10,000 spots / chip

PLEXERA ECOSYSTEM

Connect PlexArray HT
with the wider Plexera platform.

SEPARATE PRODUCTPlexJet Microarray Printer
CONSUMABLESNanoCapture Chips
SERVICEProtein Binding KineticsSERVICESPRi-SMM Screening