Project inputs
- Protein or RNA target information
- Screening objectives
- Assay requirements
- Compound library information
Services / Small-molecule kinetic screening
Screen small-molecule libraries
with real-time kinetics.
Plexera immobilizes small-molecule libraries on high-density SPRi microarrays and measures binding against protein or RNA targets across a concentration series.
Discuss your screening project- 3,840
- Compounds per chip
- ~3 hours
- Per screening run
- 8 concentrations
- Target series
- KD · kon · koff
- Real-time kinetics
A COMPLETE SCREENING SERVICE
Define the target.
Plexera runs the screen.
Complete execution
- Library layout and high-density printing
- Photocrosslinking immobilization
- Target concentration series and SPRi
- Kinetic fitting and data analysis
Ranked kinetic data
- Compound-level sensorgrams
- KD, kon and koff
- Hit ranking and clustering
- Heatmaps and a complete report
SPRi-SMM WORKFLOW
From compound library to ranked binders
The complete workflow keeps library preparation, kinetic measurement and data analysis within one coordinated project.
- 01
Prepare the library
Confirm compound composition, plate layout and project-specific printing requirements.
- 02
Print and immobilize
Print a high-density array and covalently immobilize the small molecules by photocrosslinking.
- 03
Measure the target
Run a protein or RNA target concentration series across the array and record binding in real time.
- 04
Rank the compounds
Fit kinetic parameters, compare binding behavior and organize candidate compounds for review.
SPRi-SMM ARCHITECTURE
The library stays on the chip.
The target moves through the array.
This orientation places thousands of immobilized small molecules in contact with the same protein or RNA target concentration series, creating a comparable kinetic dataset across the library.
ON THE CHIPSmall-molecule libraryCovalent photocrosslinking immobilization
+
IN THE FLOW PHASEProtein or RNA targetEight-point concentration series
=
DATA OUTParallel kinetic measurementsSensorgrams, KD, kon and koff
DATA DELIVERY
From compound library
to kinetic landscape.
Review individual sensorgrams alongside library-level ranking and clustering. The final output connects each compound to its measured binding behavior.
- Compound-level association and dissociation curves
- Affinity and kinetic parameters
- Hit ranking and response comparison
- Library heatmap and clustering
- Structured experimental report
Representative data are shown for illustration and do not constitute a performance guarantee.
SCREENING ORIENTATION
SPRi-SMM and conventional SPR
Library-scale kinetic screening
- Immobilized
- Small-molecule library
- Flow phase
- Protein or RNA target
- Measurement
- Thousands of compounds in parallel
- Role
- Screening and kinetic characterization
Selected-hit characterization
- Immobilized
- Target protein
- Flow phase
- Individual small-molecule solutions
- Measurement
- Selected compounds measured sequentially
- Role
- Detailed confirmation of prioritized hits
SCREENING APPLICATIONS
Designed for diverse
small-molecule programs.
- 01Protein-targeted screening
- 02RNA-targeted small molecules
- 03PROTAC discovery
- 04Drug repurposing libraries
- 05Fragment and focused libraries
- 06Binding selectivity profiling
PROJECT QUESTIONS
Before your screen starts
What target formats are supported?
Protein and RNA targets can be evaluated. Target quality, concentration range and assay conditions are confirmed during project planning.
Which compound library can be used?
Library composition, source, plate format and printing compatibility are reviewed for each project before the array layout is finalized.
How much compound is required?
The microarray format uses very small printing volumes. Exact sample requirements depend on library concentration, plate format and the selected chip layout.
What data are included in the report?
The report includes sensorgrams, fitted kinetic parameters, hit ranking, response comparison, clustering and the experimental context.
START A SCREENING PROJECT
Tell us about
your target and library.
Share the target format, compound library information and the screening question you want to answer.
sale@plexera.com